Two of our members of EPP from the ITQB - Cecilia M. Arraiano and Vânia Pobre - have just published this technique in Methods in Enzymology, which can be used also for Pseudomonas putida: https://authors.elsevier.com/b/1Y8Bf_1lc7dESG
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This project has received funding from the European Union’s Horizon 2020
research and innovation programme under grant agreement No 635536.